This paper explores the influence of high-speed rail construction on service integration of manufacturing enterprises from a new perspective of domestic and international double cycle. The results show that: the high-speed railway construction has a significant positive effect on the service integration of manufacturing enterprises; The construction of high-speed rail promotes the improvement of embedded service level of manufacturing enterprises, and significantly reduces the blending service integration; High-speed rail construction promotes the service integration of manufacturing enterprises through the increase of domestic market accessibility and market competition. However, the increase of international market accessibility and market competition is not conducive to the service-oriented transformation of manufacturing enterprises.