In the face of the strategic window period of accelerating the transformation of emerging technology paradigm, how to realize the systematic transcendence from catching up to leading has become an important issue for new technology enterprises. This paper takes Unitree Robotics Science and Technology as a case, integrates symbiosis theory and ambidextrous innovation theory, and constructs the theoretical framework of innovation situation-ambidextrous capability-regional symbiosis ecology-niche transition . It is found that the surpassing and catching-up of new technology enterprises follows the progressive logic of situation-driven-ability evolution-ecological shaping-potential leap. Driven by the innovation situation, enterprises combine the exploration and utilization activities in the field of technology and market in the three stages of catching-up, surpassing and leading, and construct three differentiated ambidextrous capabilities of technology following-market exploration, technology running-market expansion and technology leading-market definition, which in turn promote the regional symbiotic ecology from the form transition of point attachment-chain collaboration-network integration. Promote enterprises to achieve a continuous leap of niche niche-core niche-dominant niche. The research provides a new explanation and practical enlightenment for the dynamic adjustment of ambidextrous capability configuration of new technology enterprises.