Based on explaining the meaning of enterprise' ecological niche, the enterprises ecological niche is divided into three layers: the living force (the attribute about enterprises '" ecostate "), and the developing force (the attribute about enterprises '"ecostate "and" ecorole "), and the competition force (the attribute about enterprises ' " ecorole"). The enterprises ecological niche is also anatomized into six corresponding dimensions: the ability of production, the ability of core technology, the ability of interface management, the ability of strategic management, the ability of marketing management and the ability of learning innovation. In addition, the method is put forward for measuring the ecological niche.